High cell density cultivation of Bacillus subtilis NCIM 2063: Modeling, optimization and a scale-up procedure

Bacillus subtilis is a non-pathogenic, sporulating, gram-positive bacteria with pronounced antimicrobial and metabolic activity great potential for wide application in various fields. The aim of this paper was to determine the optimum B. NCIM 2063 growth conditions scale up biomass production from shake flasks bioreactor level. critical parameters their interaction effects were studied using Box-Bekhen experimental design response surface methodology. Developed model equations statistically significant good prediction capability. It found that during flask cultivation glucose should be added concentration 5 g l-1 DSM medium, OTR at 10 mol m-3h-1 temperature 33 ?C, achieve maximum number viable cells spores. To process level kLa used as main criterion. Scale effect evaluated by comparing kinetics laboratory bioreactor. total obtained 4.57?109 CFU ml-1 which 1.41 times higher than (3.24?109 ml-1), proving scale-up procedure conducted successfully.